Development of polyclonal antisera against movement proteins from three poleroviruses infecting cucurbits
نویسندگان
چکیده
منابع مشابه
Monoclonal and polyclonal antisera
The extensive application of the hybridoma technology for tissue phenotyping has demonstrated in the last decade that a number of heterodimeric molecules consisting of noncovalently associated α and β subunits (Hynes, 1992), named integrins, play a major role in cell-to-cell and cellto-interstitium interactions as well as in cell trafficking (Hemler, 1990; Albelda and Buck, 1990). The structura...
متن کاملSurface antigens of brain synapses: identification of minor proteins using polyclonal antisera
Antigenic proteins of brain synaptic plasma membranes (SPM) and postsynaptic densities (PSD) were characterized using antisera raised against SPM. Immunostaining of brain sections showed that the antigens were restricted to synapses, and electron microscopy revealed staining at both presynaptic terminals and PSDs. In primary brain cell cultures the antisera were also neuron-specific but the ant...
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Development of an immunoassay for clenbuterol (CLB) detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity column and the antibody purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electro...
متن کاملDevelopment and characterization of polyclonal antibody against human kappa light chain in rabbit
Polyclonal antibodies against kappa light chain are used to diagnose diseases producing free light chain. The kappa and lambda light chains are products of immunoglobulin synthesis and released into the circulation in minor amounts such as serum, cerebrospinal fluid, urine and synovial fluid in normal condition. The purpose of this study was the production and purification of polyclonal immunog...
متن کاملCharacterization of polyclonal antibodies against Japanese flounder IgM derived from recombinant IgM constant region proteins.
Cell and determinant markers are important in fish immunology and have vast applications in aquaculture but the availability of such markers are quite limited. Hence, there is a need to identify and also further improve existing markers in fish. Here, we developed effective polyclonal antibodies (pAbs) targeting specific parts of the Japanese flounder (Paralichthys olivaceus) IgM constant (C) r...
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ژورنال
عنوان ژورنال: Phytopathology Research
سال: 2020
ISSN: 2524-4167
DOI: 10.1186/s42483-020-00065-8